Detection of intestinal colonization by carbapenem-resistant Enterobacteriaceae (CRE) among patients admitted to a tertiary care hospital in Egypt

Abstract Background The irrational use of carbapenems in the last years lead to emergence carbapenem-resistant Enterobacteriaceae (CRE). This study aimed at determining prevalence CRE intestinal carriage among admitted patients a tertiary care hospital Egypt, characterize carbapenemase-producing genes and identify possible risk factors colonization. One hundred rectal swabs were collected from within 48 h admission. Culture was done on chromogenic media then identification antibiotic susceptibility testing using Vitek 2 compact system. Carbapenemase production confirmed by Rapidec Carba NP test multiplex PCR for bla OXA-48-like , NDM-like VIM-like IMP-like KPC-like . Results A total number 36 isolates recovered 28 patients. Thus, colonization 28%. Escherichia coli (83%), followed Klebsiella pneumoniae (17%) main species. History recent hospitalization prior intake statistically significant predisposing gave positive results 29/36 isolates, whereas seven negative results; six them harbored Overall, detected 24/36 (66.7%), 11/36 (30.6%) lastly 1/36 (2.8%). Conclusions Our findings confirm that is disseminating our healthcare facility, fact should be considered as pathogens causing infections high Strict infection control measures applied all carriers admission proper antimicrobial stewardship program clinical settings.